High Resolution Chromatography: A Practical Approach (Practical Approach Series)

Features

• Cover Type: Hard Cover with 336 pages
• Published by: Oxford University Press, USA
• Edition: 1st Edition July 10, 1999
• Written in: English
• ISBN 10 Number: 0199636494
• ISBN 13 Number: 978-0199636495
• Book Dimensions: 9.3 x 6.2 x 0.9 inches
• Weighs: 1.5 pounds

Product Review
"Laboratory workers will find this a useful guide to the development of effective purification protocols" Aslib Book Guide"

Product Description
Isolating and purifying proteins is a key step for a wide range biological experiments, and researchers require quick and reliable techniques. This practical volume fits this need. The first section, devoted to techniques and equipment, covers initial preparation, microscale techniques, detector technologies, and capillary electrophoresis and its associated techniques. The second section then covers a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand, while others focus on specific groups of molecules, including oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA-binding proteins.