Principles of Fluorescence Spectroscopy

Features

• Cover Type: Hard Cover with 954 pages
• Published by: Springer
• Edition: 3rd Edition September 15, 2006
• Written in: English
• ISBN 10 Number: 0387312781
• ISBN 13 Number: 978-0387312781
• Book Dimensions: 11.3 x 9 x 2.4 inches
• Weighs: 7.5 pounds

Product Review


Praise for Earlier Editions:

"Lakowicz’s Principles of Fluorescence Spectroscopy has been the best one-volume introduction to the biophysical principles of fluorescence methods.

- Roger Y. Tsien, Ph.D., Department of Pharmacology and Department of Chemistry and Biochemistry, University of California, San Diego, California

"Principles of Fluorescence Spectroscopy is encyclopedic and comprehensive."

- Britton Chance, Professor Emeritus in Biochemistry and Biophysics,University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania

"Recommended without reservation both to the novice and to the expert in fluorescence."

- Analytical Biochemistry

"In addition to its use as a student text, it should be a particularly valuable reference for those involved in biochemical research."

- Chemistry in Britain

Advance Praise for Third Edition:

"This third edition has significantly expanded the topics, and will remain as a leading reference, as well as a text…the information in the book is valuable for a wide range of disciplines."

- Robert M. Clegg, Ph.D., Department of Physics, University of Illinois, Champaign-Urbana, Illinois

"Overall this is a most welcome, and timely transformation of the classic, and most comprehensive textbook on fluorescence spectroscopy. It should be the number one item on the shopping list for any student or researcher involved in any aspect of fluorescence, be it as a biologist who does some microscopy, or a chemist synthesizing novel fluorophores."

- Alan Ryder, Ph.D., National Centre for Biomedical Engineering Science, National University of Ireland-Galway, Galway, Ireland

From the reviews of the third edition:

"This book gives an overview of the principles and applications of fluorescence. It is well structured, starting with basic knowledge about the phenomena of fluorescence and ending with the latest applications. … highly readable and informative both by novices and by experienced people. … a helpful work of reference and a wonderful creation for learning and teaching. The updated 3rd edition with its appealing design and its absolutely up-to-date and, nevertheless, complete treatment of fluorescence spectroscopy makes it essential for everyone working in this field." (Christiane Albrecht, Analytical and Bioanalytical Chemistry, Vol. 390, 2008)

Product Description


The third edition of the established classic text reference, Principles of Fluorescence Spectroscopy, will enhance upon the earlier editions' successes. Organized as a textbook for the learning student or the researcher needing to acquire the core competencies, Principles of Fluorescence Spectroscopy, 3e will maintain the emphasis on basics, while updating the examples to include recent results from the literature. The third edition also includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering.

This full-color textbook features the following:

- Problem sets following every chapter

- Glossaries of commonly used acronyms and mathematical symbols

- Appendices containing a list of recommended books which expand on various specialized topics

- Sections describing advanced topics will indicate as such, to allow these sections to be skipped in an introductory course, allowing the text to be used for classes of different levels

Principles of Fluorescence Spectroscopy, 3 rd edition, is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.



Reader Reviews
This review is from: Principles of Fluorescence Spectroscopy (Hardcover) In the sixteen years since the appearance of the first edition of Lakowicz's Principles of Fluorescence Spectroscopy, the use of fluorescence as an analytical tool has grown remarkably, especially in instrumentation, available fluorophores, and practical applications. Therefore, it shouldn't be surprising that the second edition of the work has more than doubled in size. The approach is the same as that in the first edition: fluorescence spectroscopy from the viewpoint of a biophysical chemist. Explanations are clear. References are profuse; they now number well over a thousand and include an additional appendix that cites many books and review articles for additional reading. Figures are also plentiful; no fancy color diagrams a la contemporary biochemistry texts, but basic line drawings, primarily adapted from journal articles. The first three chapters ("Introduction to Fluorescence", "Instrumentation for Fluorescence Spectroscopy", and "Fluorophores") are an excellent brief (93-page) introduction to the whole field. Most of the remaining 19 chapters are organized around the kinds of photophysical processes and spectroscopic quantities that are relevant to fluorescence. These include very thorough treatments of fluorescence lifetimes, quenching, anisotropy, and energy transfer. There are many examples drawn from journal articles. In addition, the work now covers more of the technological applications of fluorescence, especially in chapters on "Fluorescence Sensing" and "DNA Technology". These give a sense of the great contributions that fluorescence has made to biotechnology. My own work involves varied applications of fluorescence to biotechnology, and the second edition of Lakowicz is my reference book of choice. It would also be an excellent text for a graduate course on fluorescence in a chemistry or biochemistry department. All in all, this is a most worth successor to the first edition.